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A) Representative blots show protein levels analyzed by Western Blot as well as loading controls. GRK2 knockdown upregulates pERK and downregulates pIκBα. ERK and Tubulin are loading controls. B) Quantification of western blots for pERK/ERK and pIκBa/IκBa. Means +/− SEM, * p < 0.05. ** p < 0.01, compared to the corresponding siCtrl group. (n=5)

Journal: The Biochemical journal

Article Title: G-protein coupled receptor kinase-2 is a critical regulator of TNFα signaling in colon epithelial cells

doi: 10.1042/BCJ20170093

Figure Lengend Snippet: A) Representative blots show protein levels analyzed by Western Blot as well as loading controls. GRK2 knockdown upregulates pERK and downregulates pIκBα. ERK and Tubulin are loading controls. B) Quantification of western blots for pERK/ERK and pIκBa/IκBa. Means +/− SEM, * p < 0.05. ** p < 0.01, compared to the corresponding siCtrl group. (n=5)

Article Snippet: Following this transfer, the blot was probed with specific primary antibodies for pERK 1/2, total ERK-2, p38, pp38, JNK1/2, pJNK1/2 and tubulin (Santa Cruz) as well as IκBα and pIκBα (Cell Signaling) diluted in LiCor blocking buffer (LiCor) and followed by anti-rabbit or anti-mouse IR dye-conjugated secondary antibodies (LiCor).

Techniques: Western Blot, Knockdown